bodipy molecular weight

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1, 2 it co-localizes with dehydroergosterol, a marker of cholesterol, in hela cells and is trafficked from the plasma membrane to the endocytic recycling compartment in bhk cells. 2 bodipy-cholesterol … The higher apparent molecular weights of Nluc-A 3 (predicted . Synonyms: Styrene-BODIPY, Styrene-Conjugated BODIPY. The BODIPY has both strong absorption and fluorescence in the visible region; on the contrary the dipyrrolmethane has neighter absorption nor fluorescence in that region 26,27 . Still, the typical over- The BODIPY FL fluorophore produces greater fluorescence output than NBD due to its higher molar absorptivity and fluorescence quantum yield.16 The BODIPY FL fluorophore is also more photostable than NBD. The use of the fluorescence lifetime of BODIPY allows quantitative measurements of microscopic viscosity, with an extremely wide dynamic range of lifetimes, from 100 s ps to 6 ns, in the viscosity range between 1 to 10 . With respect to molecular weight, it has been suggested that rat kidney TPC1 may form a dimer to function as an ion channel (Ishibashi et al., 2000), . Purity: ≥98%. - Mechanism of Action & Protocol. [1] Upon oxidation, its excitation maximum shifts from 581 to 500 nm and the emission maximum shifts from 591 to 510 nm. Therefore, BODIPY has been used as a scaffold for fluorescent probes for various targets, candidates for solid-state solar concentrators, and materi-als for incorporation into electroluminescent devices.34-37 We previously reported a BODIPY-based molecular probe (BAP-1) with a dimethylamino styryl group that plays an 2 bodipy-cholesterol … The common name is an abbreviation for "boron-dipyrromethene". insensitivity. Prazosin is a high-affinity antagonist for the a 1-adrenergic receptor.The green-fluorescent BODIPY FL prazosin derivative can be used to localize and detect the a 1-adrenergic receptor.. It has a high extinction coefficient and fluorescence quantum yield. the BODIPY chromophore in 16 is quenched; however, we were able to observe weak fluorescence associated with the fullerene chromophore (Figure S63). It is a bright red fluorescent dye with high cell-permeability. Molecular Weight: 324.18. . Molecular Weight * = ×: ×: The dilution calculator . . Hide. Molecular Weight * = ×: ×: The dilution calculator equation . May 9-12, 2022 Booth 233. In contrast to the highly water-soluble . Synonyms: C16 BODIPY. High-resolution mass spectra of BODIPY 16 confirm its molecular composition. CAS No. With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY® 493/503 can be used as a stain for netural lipds and as a tracer for oil and Invitrogen BODIPY 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) | Fisher Scientific bodipy dyes are notable for their uniquely small stokes shifts, narrow absorption bands, sharp emissions, high fluorescence quantum yields, and excellent chemical and photostability.146,164,165 the combination of these desirable attributes makes bodipy fluorophores attractive as tools in a variety of applications, for example, in biochemical … The ranges are 30 to 170 cP for Silicone oil 100 and 165 to 850 cP for . Bodi Fluor™ 576/589 is chemically the same molecule to BODIPY™ 576/589 dye. The solution was allowed to stir at room temperature for 2 h, the product was extracted with DCM and the organic phase was neutralized with a saturated solution of Oxidation of the polyunsaturated butadienyl portion of the dye results in a shift of the fluorescence emission peak from ∼590 nm to ∼510 nm. Oxidation of polyunsaturated butadienyl portion of dye results in shift of fluorescence emission peak from ∽590nm to ∽510nm. It is interesting that under negative atmospheric-pressure chemical ionization (APCI) mode, apart from the molecular . It displays excitation/emission maxima of 558/568 nm, respectively, and has been used to monitor the localization and dynamics of lipid droplets in live cells. Catalog number: D3861. . Research Paper. Synthesis of compound 4: Compound 3 was synthesized according to the literature protocol.2 To a solution of 3 (2.1 g, 10.81 mmol) in pyrrole (10 mL) was added TFA (166 µL, 2.16 mmol, 0.2 eq) under argon. Photophysical properties of flapping peryleneimide were compared with those of representative molecular rotors, DCVJ and BODIPY as well as flapping anthraceneimide. Advanced Search. For Invitrogen BODIPY 493/503 dye (Cat. 2. Molecular Weight: 394.23. The assay is a valuable complement for drug discovery efforts and will support a better understanding of Class F GPCR pharmacology. 16 Remarkably, it was soon recognized that the properties associated to the BODIPY fluorophore, such as absorption and emission wavelength, could be easily modified by even minor structure . Molecular Weight (g/mol) Purity >95%. Polymers with BODIPY in the terminal end were synthesized by RAFT polymerization using corresponding monomers and RAFT-BODIPY. After resolubilizing in either solvent, store the stock solution frozen, desiccated, and protected from light. Results obtained from ultraviolet-visible (UV . 10006627) that displays excitation/emission maxima of 488/508 nm, respectively. We show that three guanosine 5′-triphosphate analogs with BODIPY fluorophores coupled via the gamma phosphate bind to the GTPases Cdc42, Rac1, RhoA, and Ras and displace guanosine 5′-diphosphate with high intrinsic exchange rates in the presence of Mg 2+ ions . (A, B) Spinning disk confocal images of HeLa and ORP1-null cells cultured with BODIPY-LDL. For Research Use Only. Difluor{2-[(2H-pyrr ol . Molecular Formula: C22H21BF2N2O2. Compare Product No. These low molecular weight fluorophores produce narrow absorption bandwidths with high extinction coefficients. A series of D-A dyads and D-A-D triads molecular systems based on triphenylamine and 9-ethyl-carbarzole as donor (D) and BODIPY as acceptor (A) has been designed and synthesized. It has a high extinction coefficient and fluorescence quantum yield. BODIPY aminoacetaldehyde (BAAA) is a fluorescent substrate for both murine and human aldehyde dehydrogenase (ALDH). For non-radiative decays, BODIPY dyads and triads were investigated. Molecular rotors are a subclass of intramolecular charge transfer fluorophores which form a lower energy twisted state. Synthesis Purity: >98%. This assay allows distinction of two separate binding sites for BODIPY-cyclopamine on the SMO transmembrane core in live cells in real time. Products. A new BODIPY-based long-wavelength fluorescent probe for chromatographic analysis of low-molecular-weight thiols Anal Bioanal Chem. Unlike fluorophores Invitrogen BODIPY FL C16 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic | Fisher Scientific BODIPY 581/591 undecanoic acid can be used to detect reactive oxygen species (ROS) in cells and membranes. Biological thiols, especially low molecular weight thiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play a pivotal role in physiological and pathological systems. The M n, measured by size-exclusion chromatography in THF, of S-poly and R-poly were 12 100 and 9200, respectively, and the molecular weight distributions (M w /M n) of the polymers were very wide . Compare Product No. Furthermore, the absorbance and emission spectra are . We found that the D-A-D triads exhibited broader absorption, raising . Find bodipy and related products for scientific research at MilliporeSigma. Boc Sciences offers BODIPY 530/550 C3 in bulk, please inquire us to get a quote for BODIPY 530/550 C3. . Molecular Formula: C28H43BF2N2O2. Chemsrc provides BODIPY(CAS#:138026-71-8) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Purity: >98%. Shuanglong Liu 1, Tzu-Pin Lin 2, Dan Li 1,3, Lauren Leamer 2, Hong Shan 3, Zibo Li 1 , François P. Gabbaï 2 , Peter S. Conti 1 a reversed-phase HPLC method was developed for measuring low-molecular-weight thiols including glutathione, cysteine, homocysteine, N-acetylcysteine, cysteinylglycine, and penicillamine. note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: J. Zhao thank the NSFC (21673031, 21761142005, 21911530095 and 21421005), the State Key Laboratory of Fine Chemicals (ZYTS201901), the Fundamental Research Funds for the Central Universities (DUT19TD28), Dipartimento di Scienze Chimiche, Universit{\`a} degli Studi di Padova (Visiting Scientist) for support. BODIPY Molecular Formula C23H33BF2N2O2 Molecular Weight 418.33 Quality Upgrade Quality Assurance Flexible Package Options Chemical Information Category Fluorescent Probes Molecular Formula C23H33BF2N2O2 Molecular Weight 418.33 Specification Publication citing BOC Sciences Products Bio Calculators Solution Dilution Calculator Website Search Google Search HOME . Synonyms: C16 BODIPY. BODIPY™ 581/591 undecanoic acid can be used to detect reactive oxygen species (ROS) in cells and membranes. Formula Weight: 322.2. Products. Compare Product No. We have functionalised microscope slides with BODIPY-based . The common name is an abbreviation for "boron-dipyrromethene". We show that three guanosine 5'-triphosphate analogs with BODIPY fluorophores coupled via the gamma phosphate bind to the GTPases Cdc42, Rac1, RhoA, and Ras and displace guanosine 5'-diphosphate with high intrinsic exchange rates in the presence of Mg(2+) ions, thereby . . Molecular weight: 488.5. We show that three guanosine 5′-triphosphate analogs with BODIPY fluorophores coupled via the gamma phosphate bind to the GTPases Cdc42, Rac1, RhoA, and Ras and displace guanosine 5′-diphosphate with high intrinsic exchange rates in the presence of Mg 2+ ions . To investigate whether vital staining by Nile Red or BODIPY could label both LROs and lipid droplets, we grew wild-type and glo-4(ok623) animals on OP50 E. coli diet supplemented with Nile Red or BODIPY.glo-4 encodes a putative guanine nucleotide exchange factor (GEF) for the GLO-1 Rab GTPase. The usual low solubility of BODIPY derivatives in water can be overcome by first dissolving g- or r-BODIPY in dimethylsulfoxide (DMSO), which can then be mixed with water at any ratio. BODIPY dye conjugates of low molecular weight molecules also tend to be more permeant to live cells than are conjugates of charged fluorophores (Section 14.2). Molecular Weight: 449.46. The asymmetric BODIPY 1 a (BODIPY=4,4-difluoro-4-bora-3a,4a-diaza-s-indacene), containing two chloro substituents at the 3,8-positions and a reactive 5-methyl group, was synthesized from the . The PVA containing wells were flushed three times with 1x phosphate buffered saline (PBS . MOLECULAR WEIGHT: 348.20 CAS NUMBER: N/A APPEARANCE: White to off-white solid SOLUBILITY: DMSO PURITY: ≥≥≥≥98% by HPLC STORAGE: Store at -20 ℃. C11 BODIPY 581/591 has been used in biochemical assays and live cell applications. Synthesis routes of various polymers were depicted in Schemes 1, 2 and 3, respectively. 592. Formulation: A solution in benzene. Molecular Probesʼ fluorescent sphingolipids (Figure 1) have sig nif i cant ly different spectroscopic properties. bodipy-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent bodipy group at carbon 24. Aza-BODIPY was synthesized [40,41] and used as the indicator [42], PA probe [43, 44], and photosensitizer [45]. CAS Number: 2383063-37-2. employing click chemistry. Search Within. [1], [2] Reference: [1]. {57067,57068} It has been used to monitor fatty acid uptake and metabolism in cultured cells. . 2014 Oct;406 (26):6723 . doi: 10.1091/mbc.E19-12-0697. The average molecular weight for the conjugate was 7.06±0.624 kDa, as shown in Figure 5. Protect from air and light DESCRIPTION: BODIPY-acetylene is a useful reagent to prepare BODIPY-linked conjugates of lipids, fatty acids, triglycerides, etc. Molecular Weight: 262.11. It is a red crystalline solid, stable at . The following data is based on the product molecular weight 472.4 Batch specific molecular weights . (BODIPY; Molecular Probes, Eugene, OR, USA). Regulated guanosine nucleotide exchange and hydrolysis constitute the fundamental activities of low molecular weight GTPases. . Toronto Research Chemicals 20 Martin Ross Avenue Toronto, ON Canada, M3J 2K8 International: +1 (416) 665-9696 US & Canada: +1 (800) 727-9240 Email: [email protected] TRC is a subsidiary of LGC Standards BODIPY is the technical common name of a chemical compound with formula C 9 H 7 BN 2 F 2, whose molecule consists of a boron difluoride group BF 2 joined to a dipyrromethene group C 9 H 7 N 2; specifically, the compound 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene in the IUPAC nomenclature. Description. Theranostics 2013; 3(3):181-189. doi:10.7150/thno.5984 This issue. [32,42-46] For the present BODIPY-POM dyad, TD-PBE0 allows to assign the lowest strongly dipole and spin-allowed ππ* excitations of the two BODIPY chromophores, i. e. T 13 and T 14 (at 422 nm or ~ 2.9 eV) in Figure 4b, to the sharp absorption feature measured at 501 nm. CAS Number: 1246809-50-6. The UV-vis spectrum of the unsubstituted BODIPY framework is centered in the short-wave region (470-530 nm), and this BODIPY possessed a remarkably high fluorescence quantum yield (ϕ=0.90, H 2 O). BODIPY aminoacetaldehyde consists of an aminoacetaldehyde moiety bonded to the BODIPY fluorochrome and can be used to label stem cells. The gradual accumulation of moisture in the solvent will promote aggregation over time . A novel 4,4-difuoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) copolymer with diethynylbenzene has been synthesised, and its ability to act as a photosensitiser for the photocatalytic generation of hydrogen was investigated by time-resolved spectroscopic techniques spanning the ps- to ns-timescales. In order to develop our studies, the hybrids CMPBDP@T-10 and IEP-7@T-10 were prepared from TiO 2 (PC500) and the selected CPPs based on BODIPY and BOPHY cores (10 wt% CPP), respectively (Figure 1). Emission: 508 nm. BODIPY-tamoxifen probes Tamoxifen ( Scheme 1) can block estrogen from connecting with cancer cells and directing them to grow and divide. Regulated guanosine nucleotide exchange and hydrolysis constitute the fundamental activities of low molecular weight GTPases. . The percentage weight of both CPPs was confirmed by thermogravimetric analyses TGA (see Figure S2, Supporting Information). BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. Counterstaining of all cells with DAPI was used to determine the ratio of non- Description. Regulated guanosine nucleotide exchange and hydrolysis constitute the fundamental activities of low molecular weight GTPases. Appearance: dark colored solid Molecular weight: 558.88 CAS number: 2183473-18-7 Molecular formula: C27H30N4BClF2O2S Solubility: good in DCM, DMSO, DMF Quality control: NMR 1H, HPLC-MS (95%) Storage conditions: Storage: 24 months after receival at -20°C in the dark . Articles of BODIPY are included as well. C22H30N4O √ . (Excitation/Emission: 480/508 nm). May 17-19, . . No. Bodi Fluor™ 576/589 is chemically the same molecule to BODIPY™ 576/589 dye. The controlled character of the . Property Name Property Value Reference; Molecular Weight: 705.7: Computed by PubChem 2.1 (PubChem release 2021.05.07) Hydrogen Bond Donor Count: 3: Computed by Cactvs 3.4.8.18 (PubChem release 2021.05.07) . Structure of BODIPY TR amine Absorption and emission spectra of BDP TR . Uptake of BODIPY-LDL was quantified as fluorescence intensity per cell (bar, 20 µm). D3922), one may make a stock solution of 1 mg/mL (3.8 mM) in either absolute ethanol or anhydrous DMSO. Storage-20 °C or below, protect from light. Flapping peryleneimide showed extremely high photostability and more sensitive fluorescence response in a low viscosity range (0.3-3.1 cP), which enables discrimination of . C11 BODIPY 581/591 is a lipid-soluble ratiometric fluorescent indicator of lipid oxidation. BODIPY dyes are one of the most promising classes of dyes emerging for DSC application. BODIPY dye-based ion and molecular probes can efficiently and sensitively detect many inorganic (or organic) ions and molecules. Formula Weight: 488.5. BODIPY FL LDL probe cellular . Molecular Formula: C₃₃H₃₂BF₂N₅O₆: Molecular Weight: 643.44: Category: Research Tools; Molecular Biology; Fluorescent Reagents; Dyes; Applications: BDY 650-X, SE, is a reagent that can be used as Fluorescent agent for the labeling of amines. Well-defined poly(N-isopropylacrylamide)s (PNIPAMs) with boron dipyrromethene (BODIPY) in the terminal end were prepared by reversible addition−fragmentation chain transfer (RAFT) polymerization of NIPAM with BODIPY-chain transfer agent (CTA), which was synthesized by the reaction of pyrrole and BODIPY derivative having benzyl chloride in the meso position. Applications Products Services Support. Cell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Glutathione Detection, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Nitro-Oxidative Stress Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Nitro-Oxidative . (3000e30,000 Da) and low-molecular-weight hepa-rins (LMWH) (2000e12,000 Da), which have different clinical effects, pharmacokinetics and phar-macodynamics (19). Excitation: 488 nm. This . Both transient absorption and time-resolved infrared spectroscopy were used to probe the excited . Results and Discussion 2.1. . Structure Search. These properties sometimes enhance the affinity of their ligand conjugates for receptors, as long as the overall conjugate is not too lipophilic. Formulation . Technical Data Sheet. Targeted near-infrared (NIR) optical imaging can be used in vivo to detect specific tissues, including malignant cells. BOC Sciences offers Cholesteryl BODIPY 576/589 C11 in bulk, please inquire us to get a quote for Cholesteryl BODIPY 576/589 C11. Thus, the detection of biothiols is . Its fluorescence is relatively insensitive to solvent polarity and pH change. The LMWH enoxaparin has . CAS: 1246809-50-6. conjugate 82.33 nm). BODIPY is the technical common name of a chemical compound with formula C 9 H 7 BN 2 F 2, whose molecule consists of a boron difluoride group BF 2 joined to a dipyrromethene group C 9 H 7 N 2; specifically, the compound 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene in the IUPAC nomenclature. Fluorophores were diluted to 5 nM in 1% polyvinyl alcohol (PVA, 72,000 molecular weight, MP Biomedicals, Newport Beach, CA) and 50 μl was placed per well in a 96-well glass bottom plate (Cellvis), and dried under vacuum overnight as previously described . BODIPY-Palmitate is a fluorescently tagged form of palmitic acid (Cay-10006627) that displays excitation/emission maxima of 488/508 nm, respectively. Description SDS Pricing; 790389: 99% (HPLC) Expand. STY-BODIPY is a styrene-conjugated fluorogenic probe for radical-trapping antioxidant (RTA) activity. We show that three guanosine 5'-triphosphate analogs with BODIPY fluorophores coupled via the gamma phosphate bind to the GTPases Cdc42, Rac1, RhoA, and Ras and displace guanosine 5'-diphosphate with high intrinsic exchange rates in the presence of Mg(2+) ions, thereby . Skip to content. Property Name Property Value Reference; Molecular Weight: 191.98: Computed by PubChem 2.1 (PubChem release 2021.05.07) Hydrogen Bond Donor Count: 0: Computed by Cactvs 3.4.8.18 (PubChem release 2021.05.07) Description SDS . The optoelectronic properties including optical, electrochemical, and charge carrier mobility of these molecules have been investigated. . Formulation: A solution in ethanol . It can also be used to create biosensor arrays on micro- and nano-structured surfaces . US EN. These are lower molecular weight oils with polarity identical to that of Silicone oil 1000, but with different viscosity ranges attained via temperature change between 0 and 100 °C. Thus, molecular weight of polymers with the terminated BODIPY was able to be estimated by end-group analysis method. Regulated guanosine nucleotide exchange and hydrolysis constitute the fundamental activities of low molecular weight GTPases. Herein we report the synthesis of two BODIPYs bearing an alkynyl handle for conjugation via an azide-functionalized lysine side chain to two EGFR-L1 peptide derivatives that have shown increased EGFR binding affinity and stability relative to EGFR-L1. Tuning BODIPY molecular rotors into the red: sensitivity to viscosity vs. temperature . : 194235-40-. The agents were prepared by conjugating commercially . Hence, it is called a selective estrogen receptor modulator (SERM). Molecular Formula C 9 H 7 BF 2 N 2; Average mass 191.973 Da; Monoisotopic mass 192.067032 Da; ChemSpider ID 19572352; More details: Systematic name. The triad has an extinction coefficient in the range of 200,000 M -1 cm-1, making it an extremely useful and sensitive fluorescent molecular rotor. Both LROs and lipid droplets can be vital-labelled by BODIPY fatty acid analogs. Molecular Formula: C19H17BF2N2. 1, 2 it co-localizes with dehydroergosterol, a marker of cholesterol, in hela cells and is trafficked from the plasma membrane to the endocytic recycling compartment in bhk cells. BODIPY FL Phosphatidylinositol 4,5-bisphosphate is a water soluble analog of PI(4,5)P2 labeled with the green fluorophore, Bodipy®-FL, at the sn-1 position. Synonym(s): Difluoro{2-[(3,5-dimethyl-2H-pyrrol-2-ylidene-N)phenylmethyl]-3,5-dimethyl-1H-pyrrolato-N}boron, BODIPY dye, Boron dipyrromethene fluorophore. Setareh Taki 1 and Mehdi Shafiee Ardestani 2 Author information Article notes . Surface functionalisation with viscosity sensitive dyes termed 'molecular rotors' can potentially open up new opportunities in sensing, for example for non-invasive biological viscosity imaging, in studying the effect of shear stress on lipid membranes and in cells, and in imaging contacts between surfaces upon applied pressure. BODIPY-Palmitate is a fluorescently tagged form of palmitic acid (Item No. Novel BODIPY photosensitizers were developed for imaging-guided photodynamic therapy. . Its fluorescence is relatively insensitive to solvent polarity and pH change. BODIPY 558/568 C12 is a fatty acid-conjugated fluorescent probe for lipid droplets. The green fluorescent fatty acid, BODIPY® FL C 16 can be used as a synthetic precursor to a wide variety of fluorescent phospholipids. Further studies also showed that Aza-BODIPY is an attractive platform to reach NIR absorption because the highest occupied molecular orbital (HOMO)-lowest unoccupied molecular orbital (LUMO) gap is narrowed by replacing the Figure Legend Snippet: Reduced LDL uptake in ORP1-null cells. While tamoxifen acts as an anti-estrogen in breast cells, it acts as an estrogen in other tissues, such as the uterus and bones. Excitation: 504 nm, Emission: 511 nm, Molecular Weight: 783.10 g/mol Background ER-Tracker™ Green (BODIPY ® FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). . A series of NIR fluorescent ligands targeting the prostate-specific membrane antigen (PSMA) was synthesized and each compound was tested for its ability to image PSMA+ tissues in experimental models of prostate cancer. states in BODIPY dyes. It is a red crystalline solid, stable at . These properties allow for efficient sunlight absorption and cost effect DSC construction. Molecular Weight: 809.96 : Synonyms: Cholesteryl EverFluor 576/589 C11 : Product Specification; Appearance: Solid Powder . Novel nanosized AS1411-chitosan-BODIPY conjugate for molecular fluorescent imaging. bodipy-cholesterol is a biologically active and cell-permeable analog of cholesterol that is tagged with a fluorescent bodipy group at carbon 24. Boron dipyrromethene (BODIPY) rotors are perhaps one of the most widely used class of lifetime-based molecular rotors to date. BODIPY. When fresh tissue sections were treated with the fluorescent dihydropyridine‐type Ca 2+ channel blocker, DM‐Bodipy‐DHP, . Journal: Molecular Biology of the Cell. At the same time, BODIPY dyes are widely used as fluorescent markers in protein detection, nucleic acid hybridization sequencing and disease diagnosis. The introduction of a strong electron donor to the BODIPY core through a phenyl linker combined with the twisted arrangement between the donor and the BODIPY acceptor is essential for reducing the energy gap between the lowest singlet excited state and the lowest triplet state (ΔE ST), leading to a .

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